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KMID : 0665420210360020235
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Korean Journal of Food Culture
2021 Volume.36 No. 2 p.235 ~ p.245
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Anti-proliferative and Apoptotic Activity of Extracts of Lindera glauca Blume root in Human HCT116 Colorectal Cancer Cells
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Kim Yeah-Un
Moon Ha-Rin
Han In-Hwa
Yun Jung-Mi
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Abstract
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Lindera glauca Blume has been used in Korean traditional medicine to treat the symptoms of paralysis, abdominal pain,speech disorders, extravasations, contusions, and pain caused by rheumatoid arthritis. We investigated the effect of L.
glauca Blume extracts on the proliferation of colorectal cancer cells in vitro using HCT116 human colorectal cancer cell lines.
We also investigated its mechanism of action. For this purpose, we used the MTT assay, western blotting, DNAfragmentation analysis, and flow cytometry. HCT116 cells were cultured in several concentrations of ethanol extracts of L.
glauca Blume root (0, 50, 100 μg/mL). In this study, colon cancer cell growth was inhibited by L. glauca Blume root extractin a dose-dependent manner. It was associated with induction of apoptosis as assessed by nuclear fragmentation and cellcycle analysis. Apoptosis was assessed using western blotting for TNF-α, IL-6, NF-κB, Caspase-3, PARP, Bax, Bcl-2, andSIRT1. The extract also dose-dependently upregulated the expression Bax, the pro-apoptotic gene and downregulated theexpression of the anti-apoptotic gene Bcl-2. Furthermore, the extract enhanced Caspase-3 activity in a dose-dependentmanner. Our findings provide evidence that L. glauca Blume extract may mediate its anti-proliferative effect via themodulation of apoptosis.
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KEYWORD
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Lindera glauca Blume, anti-proliferative activity, apoptosis, HCT116, colorectal cancer cells
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